TOLL-LIKE RECEPTORS, ACTIVATED BY BACTERIAL LIGANDS, IN PATHOGENESIS OF MISSED AND SPONTANEOUS ABORTIONS

Author(s):  S.P. Pakhomov, Dr., Prof., Belgorod National Research University, Belgorod, Russia

O.P. Lebedeva, Dr., associate Professor, Belgorod National Research University, Belgorod, Russia

O.N. Ivashova, Belgorod National Research University, Belgorod, Russia

I.O. Zhukova, Belgorod National Research University, Belgorod, Russia

Issue:  Volume 41, №1

Rubric:  Clinical Medicine

Annotation:  The article describes the role of Toll-like receptors, activated by bacterial ligands, in pathogenesis of missed abortions and spontaneous miscarriages of early stages pregnancy. 34 patients with missed abor-tions, 34 patients with spontaneous abortions and 57 patients with progressive pregnancy (control group, admitted for medical abortion) were examined by quantitative polymerase chain reaction (PCR). Gesta-tional age of termination of pregnancy in all cases was 6-10 weeks. Patients with endocrine diseases, se-vere extragenital disorders and antiphospholipid syndrome were excluded from research. Endometrial tissue was taken by uterine abrasion. Expression of mRNA of TLR1, TLR2, TLR4, TLR5, TLR6, TLR10 in endometrium was performed by quantitative polymerase chain reaction (PCR). In endometrium of pa-tients with missed abortions and with spontaneous miscarriages expression of mRNA of TLR6 (ligand – lipopeptides of gram-positive bacteria) was significantly lower (0.026 (0.0003; 0.128) and 0.074 (0.013; 0.129) accordingly), than in the control group (7.14 (0.15; 10.30)) (p<0.01). Expression of mRNA of TLR1, TLR2, TLR4, TLR5 and TLR10 has no significant difference compared with control group. Ex-pression of mRNA of TLR1, TLR2, TLR4, TLR5 and TLR10 in endometrium of patients with missed and spontaneous abortion also has no significant difference compared with control group. Expression of mRNA of TLR5 was 3 times higher in patients with missed abortions compared with patients with spon-taneous abortions, which may be because of the presence of flagellin-containing bacteria.

Keywords:  missed abortion, spontaneous abortion, Toll-like receptors, TLR, endometrium.

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