Identification and assay of the ketoprofen esters

Author(s):  N.A. Lyapunov, Dr., Prof., State Scientific Institution «Institute for Single Crystals» of NAS of Ukraine, Kharkiv, Ukrain, lyapunov.na@gmail.com

I.A. Zinchenko, State Scientific Institution «Institute for Single Crystals» of NAS of Ukraine, Kharkiv, Ukrain

E.P. Bezuglaya, candidate of Sciences, State Scientific Institution «Institute for Single Crystals» of NAS of Ukraine, Kharkiv, Russia

Issue:  Volume 41, № 3

Rubric:  Pharmaceutical Sciences

Annotation:  The results of studies that justify the approach to the qualitative and quantitative determination of the ketoprofen esters by liquid chromatography with diode array detection and mass spectrometry detection are provided. The analytical procedure for their qualitative and quantitative determination has been developed and solutions of ketoprofen and its esters in specific concentration ranges have been tested; the response factors and correction factors were determined to assay of esters using ketoprofen reference standard (RS). It has been shown that with the increase in the molecular weight of the ketoprofen esters, their specific absorbance values are proportionally reduced as well as the correction factors increased. For the routine determination of ketoprofen esters as related substances (impurities) each of them can be identified by the UV spectrum, the absorption maximum and the relative retention of its peak as well as quantitatively determined using кetoprofen RS taking into account the correction factor. In such case, the

Keywords:  ketoprofen, ester, impurity, chromatogram, peak, absorption spectrum, absorption maxima, molecular mass (Mr), peak purity index.

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